The first isolate of this genus, a moderate thermophile, was originally described as clostridium nigrificans werkman and weaver, 1927, later as sporovibrio desulfuricans starkey, 1938, and finally. Desulfotomaculum nigrificans atcc 19998 were kindly supplied byj. Doelle, in bacterial metabolism second edition, 1975. Desulfotomaculum definition of desulfotomaculum by medical. Desulfotomaculum thermocisternum desulfotomaculum aeronauticum desulfotomaculum halophilum desulfotomaculum kuznetsovii.
The cells of these strains were motile rods that were 1 to 1. Biotechnology to atcc valued customers, atcc stands ready to support our customers needs during the coronavirus pandemic. In this work, microbiologicallyenhanced galvanic corrosion between the carbon steel beneath a deposit and bare steel was investigated in simulated oilfieldproduced water containing sulfatereducing bacteria srb, in this case desulfotomaculum nigrificans. Here, we present its draft genome in which we found genes related with the anaerobic respiration of sulfur compounds similar to those present in the copahue environment. The first isolate of this genus, a moderate thermophile, was originally described as clostridium. Sulfatereducing bacteria that form heatresistant endospores are classified in one genus, desulfotomaculum campbell and postgate, 1965. Studies on removal of metal ions and sulphate reduction using rice husk and desulfotomaculum nigrificans with reference to remediation of acid mine drainage an article from. Desulfotomaculum copahuensis strain cindefi1 is a novel sporeforming sulfatereducing bacterium isolated from the copahue volcano area, argentina. In the presence of na 2 s 2 o 4, p582 formed complexes with co and, under alkaline conditions, pyridine. Pdf phylogenetic analysis of the genus desulfotomaculum. The results of phylogenetic analysis demonstrated that the genus desulfotomaculum already has lost the clustering monophyly and was segregated into.
They are phylogenetically very closely related and belong to subgroup a of the desulfotomaculum cluster 1. The results of a phylogenetic analysis of the 16s rrna gene indicated that these strains belong to the genus desulfotomaculum, but are distinct from previously described desulfotomaculum species. Desulfotomaculum acetoxidans widdel and pfennig atcc. The phosphoroclastic system used in this study was prepared essentially as reported for obtaining the ferredoxin free clastic system from clostridium pasteurianum. Desulfotomaculum arcticum is a sporeforming, moderately thermophilic, sulfatereducing bacterium. Desulfotomaculum article about desulfotomaculum by the. Reducing agents did not protect the vegetative cells of this.
It reacted with na2 s2 o4 and was characterized by absorption maxima in the oxidized state at 392, 582, and 700 nm. A summary of the diversity and biochemistry of bacteria that carry out a dissimilatory sulfate reduction has been given in chapter 24. In 1965, the genus desulfotomaculum was created for sulfatereducing bacteria that form heatresistant spores. Review of desulfotomaculum species and proposal of the. Sporulation was observed only when acetate was the organic substrate. The separation of an autoxidizable brown pigment, p582, from desulfotomaculum nigrificans is described.
Sodium sulfite, upon reduction, produces hydrogen sulfide. Extracts of desulfotomaculum nigrificans, desulfotomaculum orientis, and desulfotomaculum ruminis exhibit lowlevels ofinorganic pyrophosphatasebut were found to have high levels of pyrophosphate. Six strains of thermophilic, endosporeforming, sulfatereducing bacteria were enriched and isolated from 2. Physical characteristics light coloured, free flowing powder. Cells of strain gss09t were anaerobic, gramstainpositive, motile, endosporeforming and rodshaped. These include anaerobic bacteria that reduce sulfates srb, sulfatereducing bacteria 88,94,95, as desulfovibrio vulgaris, desulfuricans, fairfieldensis, gigas or desulfotomaculum nigrificans. The desulfitation is performed in such a way that the semifinished and finished products retain their original natural qualities color, odor, food value, and taste. It is not clear as yet whether the tetrapyrrolic prosthetic group of desulfoviridin is present in the enzyme as the free. Reader is wrong desulfotomaculum belongs in the clostridia.
Desulfotomaculum definition of desulfotomaculum by. Four other species were sensitive to 3 h of aeration. Desulfotomaculum is a genus of grampositive, obligately anaerobic soil bacteria. The efficacy of the bark of eucalyptus tereticornis smith as an adsorbent for the removal of metal ions and sulphate from acid mine water was assessed. Very limited knowledge is available on the potential mechanisms of metal reduction among gram. Pdf description of desulfotomaculum nigrificans subsp.
The cells grew by oxidizing h2, formate, methanol weakly, lactate incompletely, to acetate and co2. Because ofthis unique property, the pathway of glucose degradation by d. Read alternative procedures for enumeration of desulfotomaculum nigrificans spores in raw ingredients of soy protein. This digital document is a journal article from chemosphere, published by elsevier in. Based products, journal of food science on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Jan 01, 2009 the efficacy of the bark of eucalyptus tereticornis smith as an adsorbent for the removal of metal ions and sulphate from acid mine water was assessed. Desulfotomaculum nigrificans atcc 19858 designation. If the sporeforming sulfatereducing bacteria are separated from the nonsporulating types, the specific epithet desulfuricans must remain with the nonsporulating group, and so must the generic name desulfovibrio, since the latter is the present equivalent of beijerincks spirillum. Desulfotomaculum article about desulfotomaculum by the free. Desulfotomaculum nigrificans werkman and weaver campbell and postgate atcc 19858 freezedried forprofit. A genus of rodshaped straight or curved, anaerobic, chemoorganotrophic motile bacteria that stain gramnegative but have grampositive cell walls. Carbon monoxidereacting pigment from desulfotomaculum nigrificans and its possible relevance to sulfite reduction.
One of the first species that was included in this new genus was d. The results show that the srb enhance the generation of a galvanic coupling effect between bare steel and the steel under. Siroheme and sirohydrochlorin journal of biological. Biocorrosion of dental alloys due to desulfotomaculum. The results of phylogenetic analysis demonstrated that the genus desulfotomaculum already has lost the clustering monophyly and was segregated into some distinct groups with low sequence. One of the first species that was included in this new genus was. Bioremediation studies using a typical sulfate reducing bacterium namely desulfotomaculum nigrificans, after pretreatment of the. Bioremediation of acid mine water using fly ash and. The genus desulfotomaculum is a heterogeneous group of sporeforming sulfatereducing bacteria.
Growth and antioxidant activity of desulfotomaculum. Comparative bioenergetics sulfate reduction desulfovibrio and. Desulfotomaculum acetoxidans widdel and pfennig atcc 49208. This is just one example of how critical the trna is to defining codon meaning.
Microbiologicallyenhanced galvanic corrosion of the steel. About 96% of fe, 75% of zn, 92% of cu and 41% of sulphate removal was achieved from the acid mine water of ph 2. The results of phylogenetic analysis demonstrated that the genus desulfotomaculum already has lost the clustering monophyly and was segregated into some distinct groups with low sequence similarity. Desulfotomaculum species are common in anoxic freshwater, brackish or marine sediments. Desulfotomaculum nigrificans werkman and weaver 1927 campbell and postgate 1965, 361 al clostridium nigrificans werkman and weaver 1927, 63. Desulfotomaculum nigrificans werkman and weaver 1927 campbell and postgate 1965 approved lists 1980 emend. Based products, journal of food science on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at. Desulfotomaculum nigrificans definition of desulfotomaculum. Thus, we propose a new species, desulfotomaculum putei, for them, with strain th11 smcc w459 as the type strain.
Sporulation and further nutritional characteristics of. Desulfotomaculum are usually found living in underground water, inside sediment or rock, or within feces of animals mainly pigs where little oxygen has reached. Desulfotomaculum nigrificans derived from atcc 7946, sku. Review of desulfotomaculum species and proposal of the genera. Desulfotomaculum nigrificans 4, a thermophilic sulfatereducing bacterium 3, in contrast to other sulfatereducers, can be cultivated in media containing glucose as the energy source. Organic nutrition stimulation of growth or sulfide yield, or both, by materials such as yeast extract or peptone hasbeenreported often 19, 38, 43, 119, 4. Comparative bioenergetics sulfate reduction desulfovibrio. Desulfotomaculum belongs in the clostridia class of the firmicutes.
A type of sulfatereducing bacteria, desulfotomaculum can cause food spoilage in poorly processed canned foods. Acetateoxidizing sulfatereducing bacteria of the desulfotomaculum acetoxidans type have been enriched from animal manure, rumen content and dung contaminated freshwater habitats, indicating that they are primarily intestinal bacteria. Conversely, extracts ofdesulfovibrio gigas, desulfovibrio vulgaris, anddesul. Relatively few nucleotide changes in a trna can lead to. The cluster consists of phylogenetically wellseparated lineages containing i desulfotomaculum nigrificans, desulfotomaculum aeronauticum, and desulfotomaculum ruminis, ii desulfotomaculum. Desulfotomaculum acetoxidans atcc 49208 designation. Click the button below to download a pdf of our latest catalog. Separation of hydrogenase from intact cells of desulfovibrio vulgaris.
Draft genome sequence of the sulfatereducing bacterium. Desulfotomaculum nigrificans are moderately thermophilic members of the polyphyletic sporeforming genus desulfotomaculum in the family peptococcaceae. Iron sulphite agar oxoid cm79 formula tryptone grams per litre 10. In thiosulfate reductase isolated from desulfotomaculum. The authors characterized a novel trnaseclike trnacys from desulfotomaculum nigrificans that recodes uga codons with cysteine instead of selenocysteine and dubbed this new molecule trnarec to indicate its ability to recode with cysteine. Carbon monoxidereacting pigment from desulfotomaculum. The free energy of adsorption of the metal ions onto fly ash was found to be negative, while the adsorption process was endothermic and adhered to the lagergren pseudofirst order rate equation. Alternative procedures for enumeration of desulfotomaculum. Formation of spores resulted in oxygen resistance of d. Desulfitation article about desulfitation by the free.
Since the tetrapyrrolic prosthetic groups of the sulfite reductases of e. Desulfotomaculum nigrificans werkman and weaver campbell. Thiosulfate disproportionation by desulfotomaculum. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services. Description of two new thermophilic desulfotomaculum spp. Theflask was connected to an absorption train of two test tubes, eachcontaining. Desulfotomaculum ruminis desulfotomaculum nigrificans desulfotomaculum australicum desulfotomaculum thermobenzoicum desulfotomaculum geothermicum desulfotomaculum sp. Genome analyses of the carboxydotrophic sulfatereducers. The addition of acetate enhanced growth and thiosulfate disproportionation. Studies on removal of metal ions and sulphate reduction. Survival of sulfatereducing bacteria after oxygen stress. The addition of acetate enhanced growth and thiosulfate disproportionation by d. The studies of bacterial proliferation and an analysis of the chemical composition of sample surfaces were conducted. Desulfitation the removal from uncooked preserved fruit and vegetables of the sulfurous acid used in sulfitation.
Desulfotomaculum thermobenzoicum, but not desulfotomaculum nigrificans, desulfotomaculum ruminis, or desulfosporosinus orientis, grew by disproportionation of thiosulfate, forming stoichiometric amounts of sulfate and sulfide. A novel dissimilatory feiiireducing bacterium, designated strain gss09t, was isolated from a compost sample by using a solid medium containing acetate and ferrihydrite as electron donor and electron acceptor, respectively. Cell free extracts were prepared by suspending the wet cell paste in 0. To atcc valued customers, atcc stands ready to support our customers needs during the coronavirus pandemic. Half volume of the inoculum was used immediately growth and antioxidant activity of desulfotomaculum acetoxidans dsm 771 cultivated in acetate or lactate. The type species of the genus is desulfotomaculum nigrificans approved lists 1980 emend. Desulfotomaculum wikispecies, free species directory. Desulfotomaculum nigrificans derived from atcc 7946. Its type strain is 15 t dsm 17038 t jcm 12923 t references. Taken into account the absence of direct contacts of the city buildings with groundwater and low winter temperatures, the role of nonsporeforming and sporeforming but thermophilic sulfatereducing bacteria genus desulfotomaculum in destruction of houses is minimal. Akagi, anddesulfotomaculum orientis atcc 19365 was purchased from the. Desulfotomaculum acetoxidans strain dsm 771 was grown at room temperature 19. Classification of the sporeforming sulfatereducing bacteria.
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